Antigen

Antigen Class

TFs and others

Antigen

PHOX2B

Cell type

Cell type Class

Neural

Cell type

IMR-32

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

source_name

IMR32_PHOX2B_ChIPSeq

cell line

IMR32

cell type

Neuroblastoma

genotype/variation

siHAND2

chip antibody

PHOX2B (santa cruze, sc-376997 X)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody following the Active Motif's instructions of the ChIP-IT High Sensitivity Kit. Libraries were prepared according to Illumina's TruSeq ChIP Sample Preparation Guide.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

31952436

Reads aligned (%)

92.9

Duplicates removed (%)

16.5

Number of peaks

3573 (qval < 1E-05)

hg19

Number of total reads

31952436

Reads aligned (%)

92.5

Duplicates removed (%)

17.1

Number of peaks

3514 (qval < 1E-05)