Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PHOX2B

Cell type

Cell type Class
Neural
Cell type
IMR-32
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
IMR32_PHOX2B_ChIPSeq
cell line
IMR32
cell type
Neuroblastoma
genotype/variation
siHAND2
chip antibody
PHOX2B (santa cruze, sc-376997 X)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody following the Active Motif's instructions of the ChIP-IT High Sensitivity Kit. Libraries were prepared according to Illumina's TruSeq ChIP Sample Preparation Guide.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
31952436
Reads aligned (%)
92.9
Duplicates removed (%)
16.5
Number of peaks
3573 (qval < 1E-05)

hg19

Number of total reads
31952436
Reads aligned (%)
92.5
Duplicates removed (%)
17.1
Number of peaks
3514 (qval < 1E-05)

Base call quality data from DBCLS SRA